Ribonucleic acid polymerase induced by vaccinia virus: lack of inhibition by rifampicin and alpha-amanitin.
نویسندگان
چکیده
The RNA polymerase activity present in the cytoplasm of BHK cells infected with vaccinia virus is not affected by rifampicin or by alpha-amanitin.
منابع مشابه
Inhibition by alpha-amanitin of ribonucleic acid polymerase solubilized from rat liver nuclei.
1. alpha-Amanitin inhibits in vitro the RNA polymerase solubilized from isolated rat liver nuclei. 2. In contrast with previous observations with whole nuclei, the inhibition occurs approximately to the same extent in the presence and in the absence of ammonium sulphate. 3. Evidence is presented that the toxin acts by interacting with the enzyme itself and not with DNA or other components.
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1. alpha-Amanitin inhibits in vitro the activity of RNA polymerase II of rat brain nuclei. 2. The toxin does not pass the blood-brain barrier, but when injected intracerebrally is highly toxic for rats, and causes inhibition of RNA polymerase II of isolated brain nuclei. 3. Intracerebral injection of alpha-amanitin 6h before training to a passive avoidance task is followed by impaired performan...
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A DNA-dependent RNA polymerase has been extracted from vaccinia virions and purified to near homogeneity as judged by glycerol gradient sedimentation and polyacrylamide gel electrophoresis. The native enzyme has a molecular weight of approximately 500,000 and can be dissociated into putative subunits of 140,000, 137,000, 37,000, 35,000, 31,000, 22,000, and 17,000 daltons. Activity was dependent...
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ورودعنوان ژورنال:
- Journal of virology
دوره 5 2 شماره
صفحات -
تاریخ انتشار 1970